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Characteristics of recombinant trehalose synthases from mesophilic microorganisms.
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Characteristics of recombinant trehalose synthases from mesophilic microorganisms.

Journal: Journal of Microbiology and Biotechnology

Article Title: Screening, Cloning, Expression and Characterization of New Alkaline Trehalose Synthase from Pseudomonas monteilii and Its Application for Trehalose Production

doi: 10.4014/jmb.2106.06032

Figure Lengend Snippet: Characteristics of recombinant trehalose synthases from mesophilic microorganisms.

Article Snippet: The optimal temperature for this enzyme was 40°C , which was higher than that for many previously reported TreS enzymes isolated from mesophilic microorganisms as shown in , for example Pseudomonas sp. P8005, P. stutzeri CJ38, P. putida NBRI0987, C. glutamicum ATCC13032, D. radiodurans DSMZ 20539, R. opacus ACCC 41021, A. aurescens CGMCC 1.1892 and A. chlorophenolicus SK33.001, which showed optimal temperatures ranging from 25 to 37°C [ - , , , - ] but lower than those for TreS enzymes from thermophilic microorganisms such as Thermus sp., Thermobaculum sp., Picrophilus sp. and Meiothermus sp., which have an optimal temperature range between 45-65°C [ , , - ].

Techniques: Recombinant, Activity Assay